ISOLATION AND CHARACTERIZATION OF BACTERIA FROM READY _TO_ DRINK BEVERAGES (FURA DE NUNU, TIGERNUT DRINK AND SOYAMILK)

OKOSHI, JUDITH CHISOM (2025) ISOLATION AND CHARACTERIZATION OF BACTERIA FROM READY _TO_ DRINK BEVERAGES (FURA DE NUNU, TIGERNUT DRINK AND SOYAMILK). Other thesis, GODFREY OKOYE UNIVERSITY, ENUGU.

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Abstract

Ready-to-eat (RTE) beverages such as Fura de Nunu, tigernut drink and soyamilk are widely consumed in Nigeria for their nutritional value and accessibility. However, their traditional preparation and informal sale often occur under unhygienic conditions, increasing the risk of microbial contamination and posing public health concerns. A total of 20 beverage samples (Fura de Nunu, soya milk, and tigernut drink) were collected from vendors in New Artisan market, Abakpa market, Thinkers corner and New Haven market, Enugu. Standard microbiological techniques were used for bacterial isolation and identification, including serial dilution, selective media culturing, Gram staining, and biochemical tests such as catalase, coagulase, indole, citrate, oxidase and sugar fernmentation test. The study revealed varying levels of bacterial contamination across all sample types, with tigernut drink exhibiting the highest microbial load. Pathogens identified included Escherichia coli, Staphylococcus species, Klebsiella species, Citrobacter species and Enterobacter species. Many isolates were catalase-positive and oxidase- negative, consistent with Enterobacteriaceae and Staphylococcus species. The presence of foodborne pathogens in all beverage types highlights serious lapses in hygiene during preparation and handling. These findings underscore the need for improved food safety practices and regulatory oversight to protect consumers from potential health risks.

Item Type: Thesis (Other)
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 05 Jun 2026 08:37
Last Modified: 05 Jun 2026 08:37
URI: http://eprints.gouni.edu.ng/id/eprint/5753

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