MOLECULAR DETECTION OF VANCOMYCIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM COMMERCIAL SOY MILK SOLD IN THINKERS CORNER

FIDELIS, CHIAMAKA PRECIOUS (2025) MOLECULAR DETECTION OF VANCOMYCIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM COMMERCIAL SOY MILK SOLD IN THINKERS CORNER. Other thesis, GODFREY OKOYE UNIVERSITY, ENUGU.

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Abstract

Staphylococcus aureus is an important foodborne pathogen that causes major concerns to the public health especially when it becomes resistant to the important antibiotics like vancomycin. As the use of plant derived drinks such as soy milk have grown, the issue of microbial contamination and antimicrobial resistance has taken more concern particularly in regions with little regulation. The research was conducted to find out the occurrence of vancomycin-resistant Staphylococcus aureus (VRSA) in soy milk retailing at Thinker Corner, Enugu. Eight samples of soy milk were randomly purchased in the region retail outlets. The eight samples were isolated using Mannitol Salt Agar (MSA). Suspect colonies of S. aureus were Gram stained and biochemical tests was carried out in order to identify the samples on which they grew. The well diffusion method was employed in antimicrobial susceptibility testing; vancomycin was the test antibiotic. The presence of resistance genes in relation to vancomycin resistance was determined using polymerase chain reaction (PCR). Cultural, microscopic and biochemical analysis identified all the eight isolates as S. aureus. Interestingly, there was one isolate which was resistant to vancomycin. Presence of vancomycin resistance genes in the resistant isolate was confirmed by molecular analysis, which demonstrated presence of VRSA in commercial soy milk. The detection of this pathogen indicates a possible health hazard to the population and the necessity of strict hygiene standards and regular microbial testing and regulation during the production and sales of the plant-based drinks.

Item Type: Thesis (Other)
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 15 Jun 2026 10:34
Last Modified: 15 Jun 2026 10:34
URI: http://eprints.gouni.edu.ng/id/eprint/5798

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