ANTIBIOTIC RESISTANCE PATTERNS OF BACTERIA ISOLATED FROM HOSPITAL WASTE WATER

ENUKA, OKEIFUNANYA STEPHANIE (2025) ANTIBIOTIC RESISTANCE PATTERNS OF BACTERIA ISOLATED FROM HOSPITAL WASTE WATER. Other thesis, GODFREY OKOYE UNIVERSITY, ENUGU.

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Abstract

This study investigated the antibiotic resistance patterns of bacteria isolated from hospital wastewater. Two water samples were taken from two separate points at the effluent discharge area of a private hospital. The physical and chemical parameters of the wastewater, including pH, electrical conductivity, and total solids were determined. The samples were then subjected to presumptive tests for coliforms as well as determination of total bacterial counts. A series of microbiological and biochemical tests were conducted to identify and characterize bacterial isolates from the samples. The results showed the pH of the samples to be neutral; the total solids were within range and the conductivity was 422 s/m. The total bacterial counts ranged from 1.5 x 10 2 CFU/ml to 2.12 x 10 2 CFU/ml. The bacterial isolates included various pathogenic bacteria, notably Bacillus spp, Staphylococcus spp, Escherichia coli, Pseudomonas spp and Salmonella spp. Antibiotic susceptibility testing showed a high level of resistance to commonly used antibiotics such as Ampicillin, Levofloxacin, Ofloxacin, Ciprofloxacin, Gentamicin and Erythromycin. Some isolates showed intermediate sensitivity to gentamicin and ciprofloxacin, while resistance to Levofloxacin, Ofloxacin and Ciprofloxacin was also observed. The Multiple antibiotic resistance index of the isolates ranged from 0.5-1. The presence of multi-drug resistant strains highlights the public health risks associated with improper wastewater management in healthcare environments. This underscores the urgent need for effective treatment of hospital effluents before discharge into the environment.

Item Type: Thesis (Other)
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 03 Jun 2026 10:32
Last Modified: 04 Jun 2026 08:27
URI: http://eprints.gouni.edu.ng/id/eprint/5736

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