IDENTIFICATION AND CONSERVATION OF INDIGENOUS MEDICINAL PLANTS FOUND IN SELECTED LGA’S IN ENUGU STATE USING DNA BARCODING.

EKEOGBEDE,, EBUKA FAVOUR (2023) IDENTIFICATION AND CONSERVATION OF INDIGENOUS MEDICINAL PLANTS FOUND IN SELECTED LGA’S IN ENUGU STATE USING DNA BARCODING. Other thesis, GODFREY OKOYE UNIVERSITY, ENUGU.

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Abstract

This research work DNA Barcoding of Traditionally Used Medicinal Plants in Enugu State, was aimed at cataloguing the various medicinal plant biodiversity available within reach in some LGA of Enugu state. The use of plants as medicine was practiced by our ancestors, a process which must have started by trial and error until now. Twenty-four samples of medicinal plants were gathered from the three prominent towns in Enugu state: Akautara uwenu inNsukka, Ngwo, and Ugwuomu-Nike. A gene region in the chloroplast rbcl gene was amplified through PCR using rbcl primers after DNA from the medicinal plants was extracted using the silica resin technique. A considerable amount of the DNA was successfully recovered from the gel after the agarose gel electrophoresis was complete. Though all the DNA was retrieved, some were more apparent than the others. The PCR amplicons were validated using agarose gel electrophoresis, 66.7% (16) samples had clear DNA bands, 16.7% (4) samples had faint DNA bands, while 16.7% (4) samples had no DNA bands, after gel electrophoresis section, after that, the amplicons were transferred to Genewiz INC. for sequencing.The samples were analyzed using the blue line DNA Barcoding pattern of the DNA Subway and National Center for Biotechnology Information (NCBI). After analysis, 16.67 % (4 samples) had new polymorphisms, 50.00% (12 samples) were existing records and 20.83% (5 samples) needs identification. The preservation of traditional knowledge pertaining to the utilization and preservation of medicinal plants holds great significance, as these groups possess invaluable knowledge that contributes to scientific progress and the application of DNA barcoding approaches for the identification and conservation of these medicinal plants.

Item Type:	Thesis (Other)
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Natural and Applied Sciences
Depositing User:	Uchenna Eneogwe
Date Deposited:	02 Jun 2026 10:13
Last Modified:	02 Jun 2026 10:13
URI:	http://eprints.gouni.edu.ng/id/eprint/5721

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