ANTIBIOTIC RESISTANCE PROFILE AND ESBL PRODUCTION IN Escherichia coli ISOLATED FROM FROZEN FISH IN ENUGU STATE.

IBEGBULEM, UCHENNA DAVID (2025) ANTIBIOTIC RESISTANCE PROFILE AND ESBL PRODUCTION IN Escherichia coli ISOLATED FROM FROZEN FISH IN ENUGU STATE. Other thesis, GODFREY OKOYE UNIVERSITY..

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Abstract

The rise in the incidence of antimicrobial resistance (AMR) mediated by extended-spectrum beta-lactamase (ESBL)-producing bacteria in food, poses a consequential risk to the public health sector. This study investigated the patterns surrounding antimicrobial resistance and ESBL production in Escherichia coli isolated from frozen fish sold in markets across Enugu State, Nigeria. Thirty fish samples were collected from Abakpa, Eke Emene, and Old Park markets. Standard microbiological techniques were employed for isolation, identification, and biochemical characterization of E. coli. Antimicrobial susceptibility was evaluated using the Kirby-Bauer disk diffusion method, and ESBL production was confirmed phenotypically. A total of 16 E. coli isolates were identified, with 68.8% exhibiting ESBL activity. Antibiotic susceptibility results showed high resistance to piperacillin/tazobactam (87.5%) and ceftazidime (68.8%), while ciprofloxacin was the most effective, with 81.3% susceptibility. Multiple Antibiotic Resistance Index (MARI) values ranged from 0.00 to 1.00, indicating varying exposure to high-risk contamination sources. Although ESBL-positive isolates exhibited higher MARI scores, statistical analysis showed no significant difference between ESBL-positive and negative groups (p=0.68). These results indicates that frozen fish sold in markets within Enugu metropolis harbor multi-drug-resistant Escherichia coli, therefore, there is need for awareness for better hygiene in fish handling, proper use of antibiotics in aquaculture, and regular monitoring of resistance to protect public health and ensure food safety.

Item Type: Thesis (Other)
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 31 May 2026 00:14
Last Modified: 31 May 2026 00:14
URI: http://eprints.gouni.edu.ng/id/eprint/5687

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