Antimicrobial sensitivity pattern of urinary tract isolates from paraplegic and quadriplegic patients from National orthopaedic hospital Enugu

Okoro,, Benedict C. Antimicrobial sensitivity pattern of urinary tract isolates from paraplegic and quadriplegic patients from National orthopaedic hospital Enugu. None. (Unpublished)

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Abstract

ABSTRACT The Isolation, Identification, and Antimicrobial sensitivity pattern of pathogens isolated from urinary tracts of Paraplegia and Quadriplegia patients at National Orthopaedic Hospital, Enugu was conducted on fifteen urine samples. Urine culture was done using Cysteine Lactose electrolyte deficient agar (CLED), Mac-Conkey agar, and Blood agar and incubated at 37oC for 24 hours. The urine samples were screened for albumin presence using Combi-2 urinalysis dipsticks. Four microorganisms were isolated and identified by means of biochemical testing which included Gram stain, Oxidase test, Sugar fermentation test, IMVIC, motility test, Catalase test, Urease test and Coagulase test. Of the four (4) isolates, two were Gram-positive; one being Enterococcus Spp. with cocci shape while the other Cornyebacterium Spp. was rod shaped. The other two isolates were Gram-negative members of the Enterobacteriaceae family (Escherichia coli and Proteus Spp.). Enterococcus Spp. had the highest prevalence rate of 6 (37.5%); E. coli had the second highest frequency of occurrence at 5 (31.25%), Cornyebacterium Spp. had a lower rate occurrence at 3 (18.75%), followed by Proteus Spp. with the lowest rate of occurrence at 2 (12.25%). Antimicrobial susceptibility assay was carried out on the isolates. E. coli and Enterococcus Spp. had the highest resistance pattern; Cornyebacterium Spp. had a lower resistance pattern while Proteus Spp. had the lowest resistance pattern to the antibiotics. The Enterococci Spp. was susceptible to drugs like Amikacin, Chloramphenicol and Tetracycline. E. coli was susceptible to drugs like Chloramphenicol, Amikacin and Ciprofloxacin. Cornyebacterium Spp. was susceptible to Amikacin, Chloramphenicol, Ciprofloxacin and Tetracycline. Proteus Spp. was Ceftriaxone, Amikacin, Chloramphenicol, Ciprofloxacin, Sulfamethoxazole trimethroprim and tetracycline. All isolated microorganisms were generally sensitive to Amikacin and Chloramphenicol antibiotics showing thus to be active in-vitro.

Item Type:	Article
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Natural and Applied Sciences
Depositing User:	Okoro Benedict Chidiogor Okoro
Date Deposited:	31 Jul 2018 21:54
Last Modified:	31 Jul 2018 21:54
URI:	http://eprints.gouni.edu.ng/id/eprint/343

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