Udochukwu, U. and Chikezie,, Chidimma C. and Olannye, G.A (2017) Comparative Biosurfactant Production by Actinomycetes Isolated from Hydrocarbon Contaminated Soils, Plastic-Enriched Composting Soil and Ikpoba River Sediments in Benin-City. Nigerian Journal of Microbiology, 31 (1). pp. 3859-3866.
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Abstract
This study examined the production of biosurfactants by Actinomycetes isolated from hydrocarbon contaminated soils, plastic-enriched composting soil and Ikpoba river sediments. Soil samples were collected from some mechanic workshops in Benin City, from the Edo state waste management dumpsite located at Iyowa in Benin City and from Ikpoba River. Isolation of Actinomycetes was done using starch casein agar incorporation with antibiotics incubated for 7 – 10 days at 30oC. Growth on mineral salt medium initiated the production of biosurfactants which was extracted by centrifugation and filtration followed by liquid extraction using chloroform: methanol (2:1v/v). Characterization and stability studies were conducted. From the physico-chemical analyses, the pH of the hydrocarbon contaminated soil was 4.92±0.049; the plastic composted soil had 5.46±0.08 while that of Ikpoba river sediments was 6.62±0.056. The hydrocarbon contaminated soils had the highest concentration of iron (29.97±0.25), the plastic composted soil had the highest value for electricity conductivity (103.88±2.70) while the highest concentration for nitrate was recorded at Ikpoba River (8.34±0.436mg/kg). The isolates were screened for their ability to produce biosurfactant. The biosurfactant produced were screened for its emulsification activity and surface tension reduction ability by subjecting them to varied temperatures, pH and salt concentration. The biosurfactant was found to have a better surface tension of 117.01±0.20 dynes/cm with temperature, 160.04±0.33dynes/cm and 111.00±0.00dynes/cm for the hydrocarbon polluted soil which the highest values. The percentage emulsification activity of the biosurfactant produced from the Ikpoba river sediments recorded the highest value with respect to pH and temperature which were 38.04±0.0 and 32.40±0.00 (%). The hydrocarbon polluted soil recorded the highest percentage emulsification activity (39.01±0.04%) with salt concentration. Aerobic Gram positive rodswithextensive branching were observed confirming growth of Streptomyces sp. based on morphological and biochemical test.
| Item Type: | Article |
|---|---|
| Subjects: | L Education > L Education (General) Q Science > Q Science (General) |
| Divisions: | Faculty of Natural and Applied Sciences |
| Depositing User: | miss ifeoma agbo |
| Date Deposited: | 16 Jul 2019 09:13 |
| Last Modified: | 16 Jul 2019 09:13 |
| URI: | http://eprints.gouni.edu.ng/id/eprint/1703 |
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