Production of cellulase enzyme

Aneke, Chidera Nnamdi (2018) Production of cellulase enzyme. Other thesis, Godfrey Okoye University Ugwuomu-Nike, Enugu.

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Natural by-product of agricultural waste can be turned to products of commercial interests such as ethanol, glucose and single cell protein. Much effort from scientists and researches around the world has been  put to extend the full use of agricultural waste. Reports of cellulase enzyme production from the bioconversion of lignocellulosic materials has much been made. However, there is still much space to find the most suitable condition. The purpose of this research was to investigate the effect  of temperature, pH, different buffer solutions on Aspergilus cellulase.  In this research, Cyperus esculentus bagasse which is used as substrate is subjected to physical treatment using milling and grinding. After that, it is used as substrate in the production of cellulase enzyme using Aspergilus species fungus. Cellulase enzyme production was studied using solid state fermentation under room temperature. The production was run in a lab scale using fermentation trays. After that the enzyme was assayed at different temperatures. The optimum temperature of this cellulase was 50ºC with activity of 0.0599 ± 0.0042mol. The optimum pH for Aspergilus cellulose was 0.0636 ± 0.0159mol. Distilled water recorded the highest activity with 0.0431 ± 0.0003mol on the effects different buffer solution.  Effects of metallic ions on the Aspergillus species cellulase were investigated. Heavy metals such as Zinc and magnesium reduced the specific activity of cellulase from 0.0385 ± 0.0006 to 0.0271 ± 0.0014mol, 0.0313 ± 0.0043mol respectively. Manganese, and iron at 10mM concentration were able to increase the Aspergillus species cellulase activity from 0.0385 ± 0.0006 and 0.0445 ± 0.0003 and 0.0406 ± 0.0001mol respectively.

Item Type: Thesis (Other)
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > R Medicine (General)
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Mr. Chidera Aneke
Date Deposited: 08 Oct 2018 11:04
Last Modified: 08 Oct 2018 11:04

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