CLEMENT, MARVELLOUS YESOKO (2026) IN VITRO ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF CHITOSAN SYNTHESIZED SILVER NANOPARTICLES. Other thesis, Godfrey Okoye University, Enugu.
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Abstract
Antimicrobial resistance and oxidative stress due to free radicals are emerging critical global health issues and the development of eco-friendly biomaterials having antimicrobial and antioxidant activities has been the need of the hour. One of the most promising biomaterials for these applications is a biodegradable biopolymer, chitosan, derived from waste materials of the shells of crabs. The purpose of this study was to obtain chitosan from crab shell waste and synthesize chitosan-based nanoparticles with ascorbic acid as reducing agent. The powdered crab shell was subjected to demineralization, deproteinization and deacetylation to obtain chitosan with a 9.42% yield of 4.71 g in 50 g of powdered crab shell. All the synthesized nanoparticles were characterized by the Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS). The characteristic functional groups of chitosan were confirmed by the FTIR spectra, while the XRD spectra showed that the synthesized material was crystalline. The SEM images showed that the morphology is irregular, rough, heterogeneous and sheet like while the EDS spectrum showed silicon as the major element followed by aluminium, oxygen, magnesium, carbon and some traces of other elements. The antioxidant activity was tested by DPPH, Ferric Reducing Antioxidant Power (FRAP), Nitric Oxide scavenging and Total Antioxidant Capacity assays with maximum activities of 83.05%, 131.51%, 43.30%, and 86.35%, respectively. The results of the antimicrobial activity tests showed concentration-dependent inhibition against Staphylococcus aureus and Escherichia coli, with more activity against Staphylococcus aureus. The study results show that crab shell derived chitosan based nanoparticles have shown that it is having potential biomedical and antimicrobial properties.
| Item Type: | Thesis (Other) |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Faculty of Natural Sciences and Environmental Studies |
| Depositing User: | MICHAEL MADUBUKO |
| Date Deposited: | 07 Aug 2026 10:22 |
| Last Modified: | 07 Aug 2026 10:22 |
| URI: | http://eprints.gouni.edu.ng/id/eprint/6217 |
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