BIOAUGMENTATION STRATEGIES TO ENHANCE METHANE PRODUCTION FROM LIGNOCELLULOSIC SUBSTRATES: DYNAMICS OF THE PROKARYOTIC COMMUNITY STRUCTURE

Obi, Linda U. and Tekere, Memory and Roopnarain, Ashira and Adeleke, Rasheed A (2022) BIOAUGMENTATION STRATEGIES TO ENHANCE METHANE PRODUCTION FROM LIGNOCELLULOSIC SUBSTRATES: DYNAMICS OF THE PROKARYOTIC COMMUNITY STRUCTURE. In: 30th European Biomass Conference and Exhibition, 9-12 May 2022, Online.

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Abstract

Optimization of anaerobic digestion (AD) of lignocellulosic substrates via bioaugmentation for enhanced biomethane production is imperative. Here, eight different screened bacterial isolates were used to augment different batch anaerobic digesters that comprised freshly chopped water hyacinth (WH) and cow dung (CD), at 2% total solids (w/v). Methane production was monitored at intervals and DNA metabarcoding of the 16S rRNA genes revealed the dynamics of the prokaryotic community structure of the anaerobic digesters. Obtained results suggest bioaugmentation improved the abundance and diversity of the prokaryotic community. The treatment that was inoculated with Serratia marcescens produced the highest cumulative methane of 0.68 L, 45.6% methane more than the consortium treatment. A shift from Pseudomonas to Bacteroides was observed in the bacterial community at the genus taxonomic level after AD while Methanosarcina dominated the archaeal genera. Furthermore, independent bioaugmentation of AD of different substrates such as water hyacinth, cow dung, and cellulose powder with identified model bioaugmentation agent, Serratia marcescens portrayed a positive effect with regards to methane production when compared with the control treatment. This study represents an economic and environmentally friendly approach of single isolate inoculation (bioaugmentation) in optimizing methane production from lignocellulosic substrates such as water hyacinth.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > Q Science (General)
Divisions: Faculty of Engineering, Science and Mathematics > School of Geography
Depositing User: Uchenna Eneogwe
Date Deposited: 10 Sep 2025 12:39
Last Modified: 10 Sep 2025 12:39
URI: http://eprints.gouni.edu.ng/id/eprint/5502

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