Joshua, Parker Elijah and Anosike, C. Joy and Asomadu, Rita O. and Ekpo, Daniel Emmanuel and Uhuo, E. N. and Nwodo, Okwesili Fred Chiletugo (2020) Bioassay-guided fractionation, phospholipase A2-inhibitory activity and structure elucidation of compounds from leaves of Schumanniophyton magnificum. Pharmaceutical Biology, 58 (1). pp. 1078-1085.
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Bioassay-guided fractionation phospholipase A2-inhibitory activity and structure elucidation of compounds from leaves of Schumanniophyton magnificum.pdf Download (1MB) |
Abstract
Context: Schumanniophyton magnificum Harms (Rubiaceae) is used traditionally in Nigeria for the treatment of snake bites. Snake venom contains phospholipase A2 (PLA2) which plays a key role in causing inflammation and pain. Objective: To assess the anti-inflammatory effect of the methanol extract of Schumanniophyton magnificum (MESM) leaves through the inhibition of PLA2 and investigate the compounds responsible for the effect. Materials and methods: PLA2-inhibitory activity of MESM was assessed at concentrations of 0.1–0.8 mg/ mL using human red blood cells as substrate. Prednisolone was used as the standard control. MESM was subsequently partitioned using n-hexane, dichloromethane, ethyl acetate and aqueous-methanol (90:10 v/ v), after which PLA2-inhibitory activity of the partitions was determined. The best partition was subjected to chromatographic techniques and the fractions obtained were assessed for PLA2 inhibition at 0.4 mg/ mL. Compounds in the most active fraction were determined using Fourier-transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS). Results: MESM significantly inhibited PLA2 activity at 0.8 mg/mL (44.253%) compared to prednisolone (35.207%). n-Hexane partition (SMP1) proved more active with inhibition of 55.870% observed at 0.1 mg/ mL. Fraction 1 (SMF1) showed the highest PLA2-inhibitory activity of 58.117%. FTIR studies revealed the presence of some functional groups in SMF1, and GC-MS confirmed the presence of 9 compounds which are first reported in this plant. Hexadecanoic acid, ethyl ester was identified as the major compound (24.906%). Discussion and conclusions: The PLA2-inhibitory activity of MESM suggests that its compounds may be explored further in monitoring anti-inflammatory genes affected by the venoms.
Item Type: | Article |
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Subjects: | Q Science > QD Chemistry |
Divisions: | Faculty of Natural and Applied Sciences |
Depositing User: | Uchenna Eneogwe |
Date Deposited: | 28 Aug 2025 10:56 |
Last Modified: | 28 Aug 2025 10:56 |
URI: | http://eprints.gouni.edu.ng/id/eprint/5352 |
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