EVIDENCE-BASED PREFERENTIAL IN VITRO ANTISICKLING MECHANISM OF THREE NATIVE NIGERIAN PLANTS USED IN THE MANAGEMENT OF SICKLE CELL DISEASE

Ijoma, K.I and Ajiwe, V.I.E and Ndubuisi, Juliana Onyema (2022) EVIDENCE-BASED PREFERENTIAL IN VITRO ANTISICKLING MECHANISM OF THREE NATIVE NIGERIAN PLANTS USED IN THE MANAGEMENT OF SICKLE CELL DISEASE. MALAYSIAN JOURNAL OF BIOCHEMISTRY & MOLECULAR BIOLOGY, 3. pp. 9-17.

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Abstract

Sickle cell anemia is a hereditary genetic disease caused by the substitution of glutamic acid by valine at beta six (β-6) position of the hemoglobin; the clinical implication is that the erythrocyte contains the hemoglobin polymerizes, leading to severe clinical consequences. Therefore, we investigated the antisickling mechanism of Ficus thonningii (FTH), Jatropha tanjorensis (JTR) and Justicia carnae (JCN) native to Southeast Nigeria used in the management of sickle cell disease. The experiment was designed to include erythrocyte fragility, erythrocyte reversibility and polymerization inhibition mechanisms. The results obtained were analyzed using one-way ANOVA and Tukey Posthoc test. From the results of the erythrocyte fragility test, it was observed that at 0.35% saline concentration, FTH, JTR and JCN extracts reduced hemolysis to 26.548±0.056%, 18.055±0.064%, and 20.217±0.035% respectively while hydroxyurea (control drug) reduced hemolysis to 14.459±0.040%. In the presence of the control, the percentage number of sickle cells was 91.001±0.170% whereas, hydroxyurea was 30.414±0.162% while, extracts of FTH, JTR and JCN reduced sickling to 49.818±0.082%, 41.001±0.413% and 33.957±0.062% respectively. Also, the results of the polymerization inhibition analysis showed that extracts of FTH, JTR and JCN had relative percentage polymerization inhibition of 76.888±0.042%, 48.723±0.113% and 75.447±0.063%, respectively, in comparison to hydroxyurea which inhibited sickle erythrocyte polymerization by 70.903±0.150%. The results of our findings suggest the preferential but variable antisickling mechanism of the studied extracts; hence, the leaves of the assayed plants contain potential antisickling phytochemicals and should be explored further for their antisickling benefits.

Item Type:	Article
Subjects:	R Medicine > RZ Other systems of medicine
Divisions:	Faculty of Natural and Applied Sciences
Depositing User:	Uchenna Eneogwe
Date Deposited:	04 Jun 2025 11:12
Last Modified:	04 Jun 2025 11:12
URI:	http://eprints.gouni.edu.ng/id/eprint/4747

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