Antimicrobial Analysis and Structural Elucidation of Active Compounds of Nauclea latifolia Stem Extract (Pin Cushion Tree)

Ijoma, K.I and Ajiwe, V.I.E and Alisa, C.O (2017) Antimicrobial Analysis and Structural Elucidation of Active Compounds of Nauclea latifolia Stem Extract (Pin Cushion Tree). American Journal of Chemistry and Applications, 4 (2). pp. 21-26. ISSN 2381-4535

[img] Text
7110235.pdf
Download (240kB)

Abstract

Antimicrobial analysis and structural elucidation were carried out on the purified stem extract of Nauclea latifolia. The Harbone method was used for the extraction. The extracts were separated using a combination of column chromatography and thin layer chromatography, which gave rise to the isolation of two fractions; these fractions were further purified using recrystallization. The melting point of each pure fraction was determined. The purified extracts were subjected to structural elucidation using various spectroscopic techniques which include; FTIR, UV, H1 NMR, C13 NMR, DEPT1350, COSY, TOCSY,HMBC and HSQC. The spectral analysis suggested the presence of Myristic acid and Palmitoleic acid. The antimicrobial analysis (anti fungal and anti bacterial analysis) using the punched agar diffusion method was carried out on the isolated fractions comparatively with a standard drug Funbact-A cream (a broad spectrum antibiotic). A total of thirteen test organisms were used for this analysis amongst which were ten bacteria test organism and three fungi test organisms. The results from the average diameter zones of inhibition, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentrations (MFC) showed that all the fractions were all active on the entire test organism with zones of inhibition ranging from 10mm-26mm comparatively. None of these fractions showed similar anti microbial effect as the standard drug Funbact-A cream but individually could serve as antimicrobial to diseases caused by these test organisms from their MIC, MBC and MFC.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 04 Jun 2025 10:43
Last Modified: 04 Jun 2025 10:43
URI: http://eprints.gouni.edu.ng/id/eprint/4746

Actions (login required)

View Item View Item
GOUNI Repository is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.