Membrane Stabilization, Albumin Denaturation, Protease Inhibition, and Antioxidant Activity as Possible Mechanisms for the Anti-Inflammatory Effects of Flavonoid-Rich Extract of Peltophorum pterocarpum (DC.) K.Heyne (FREPP) Stem Bark

Enechi, Osmund Chukwuma and Okeke, Emmanuel Sunday and Nwankwo, Nicodemus E. and Nweze, John E. and Obilo, Chukwuebuka P. and Okoye, Christopher I. and Awoh, Ogochukwu E. (2020) Membrane Stabilization, Albumin Denaturation, Protease Inhibition, and Antioxidant Activity as Possible Mechanisms for the Anti-Inflammatory Effects of Flavonoid-Rich Extract of Peltophorum pterocarpum (DC.) K.Heyne (FREPP) Stem Bark. Tropical Journal of Natural Product Research, 4 (10). pp. 812-816. ISSN 2616-0692

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Abstract

The ever increasing discovery of large number medicinal plants and the screening of their bioactivity to provide data that will help physicians and patients make wise decision before using them. The study evaluated the membrane stabilization, albumin denaturation, protease inhibition and antioxidant activities as possible mechanisms for the anti-inflammatory effects of the stem-bark flavonoid-rich extract of Peltophorum pterocarpum (FREPP). The antioxidant activities were assessed using the hydrogen peroxide, nitric oxide (NO) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-inflammatory effect of FREPP was determined using the membrane stabilization model, albumin denaturation, protease inhibitor assay, while the total flavonoid was examined using the aluminium chloride colorimetric test. At 0.1, 0.4, 0.8 mg/mL, FREPP inhibited hypotonicity-induced haemolysis by 61.03, 76.84 and 83.84%, respectively. FREPP was effective in inhibiting albumin denaturation in a concentration-dependent manner with the highest inhibition of 69.89% at 50 µg/mL. Protease activity was significantly (P < 0.05) reduced at all concentrations compared to the control. The results showed that FREPP has antioxidant and anti-inflammatory activities.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 02 Jun 2025 09:45
Last Modified: 02 Jun 2025 09:45
URI: http://eprints.gouni.edu.ng/id/eprint/4720

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