Bacteriological Quality Assessment and Molecular Detection Of Antibiotic Resistant Genes Present in Bacteria Isolated from Ready-To-Eat Rice Sold in Selected Markets in Benin City, Nigeria

Olisaka, Frances N. and EHIAGHE, J.I and Odigie, O.O and Maduka, N. and Alabi, G.A and OSABOHIEN, G. (2021) Bacteriological Quality Assessment and Molecular Detection Of Antibiotic Resistant Genes Present in Bacteria Isolated from Ready-To-Eat Rice Sold in Selected Markets in Benin City, Nigeria. BIU Journal of Basic and Applied Sciences, 6 (2). pp. 228-254. ISSN 2563-6424

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Abstract

The spread of antibiotic resistance genes among foodborne pathogens and increasing number of cases of foodborne diseases is a threat to public health. In this study, bacteriological quality assessment of eighteen randomly sampled ready-to-eat rice wrapped with cellophane and leaf (Thaumatococcus daniellii) obtained from Okhra, Santana and Oba markets were carried out using Standard microbiological methods. The antibiotic resistance profile of bacterial isolates from the samples were tested using eleven commonly used antibiotics; detection of antibiotic resistant genes involved the use of Molecular methods. Total aerobic plate count, Escherichia coli and Staphylococcal plate count of the leaf-wrapped samples(LWS) is within the range of 5.38-5.56, 5.0-5.87 and 3.66- 5.08 log10CFU/g while the corresponding values for cellophane-wrapped samples (CWS) is 4.9-5.26, 4.46-7.15 and 4.26-5.65 log10CFU/g, respectively. Percentage occurrence of bacterial isolates from LWS include Staphylococcus aureus (21.66%), Klebsiella sp. (15 %), E. coli (43.34 %) and Bacillus cereus(20%) while the corresponding values were 20.4, 8.16, 44.8 and 10.2 % for CWS, respectively. Pseudomonas sp. (16.44 %) occurred only in the rice samples wrapped with cellophane. All the isolates demonstrated antibiotic resistance to ceftazidime, augmentin, cloxacillin, erythromycin and ceftriaxone. Aminoglycoside resistant genes- aac(6) and ant(4) were detected in the isolates. Biofilm forming activity involved 37.29 % and 31.71 % of the isolates from LWS and CWS, respectively. Consumption of RTE rice sold in the three markets could result in abdominal pain, vomiting, gastroenteritis, diarrhea, dysentery and pneumonia. More worrisome is the fact that some of the antibiotics might not provide effective treatment against food-borne illness caused by the bacteria isolated from the samples. Therefore, regular monitoring of food vendors by relevant agencies, implementation of good manufacturing practices (GMPs), and increase in level of public awareness on food safety and health implications associated with indiscriminate use of antibiotics are recommended.

Item Type:	Article
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions:	Faculty of Natural and Applied Sciences
Depositing User:	Uchenna Eneogwe
Date Deposited:	27 May 2025 13:27
Last Modified:	27 May 2025 13:28
URI:	http://eprints.gouni.edu.ng/id/eprint/4658

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