Isolation of Multiple Antibiotic Resistant Bacterial Species from Midstream Urine Samples Obtained From Consenting Healthy Female University Students In Benin City, Edo State

Odigie, O.O and OBAYAGBONA, O.N and Olisaka, Frances N. (2021) Isolation of Multiple Antibiotic Resistant Bacterial Species from Midstream Urine Samples Obtained From Consenting Healthy Female University Students In Benin City, Edo State. BIU Journal of Basic and Applied Sciences, 6 (2). pp. 218-228. ISSN 2563-6424

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Abstract

Culturable bacterial flora associated with clean catch midstream urine samples was determined using pour plate procedure. The antibiogram profile of the characterized bacterial cultures was evaluated using disc diffusion technique. The antibiotic discs utilized were; Ciprofloxacin (CIP), Cloxacillin (OB), Ofloxacin (OFX), Erythromycin (ERY), Caftazidime (CAZ), Gentamicin (CN), Cefuroxime (CXM), Ceftriaxone (CTR) and Amoxcillin Clavulanate (AM). Urine samples were obtained from thirty (30) randomly selected female students residing in two (2) female hostels located within the main campus of a private university in Benin City, Edo State. The heterotrophic bacterial count on Nutrient agar and Blood agar plates for the urine samples varied from 5 cfu to 263 cfu and 2 cfu to 220 cfu respectively. The observed difference between the urine derived bacterial counts was statistically significant (p<0.05). The differential bacterial counts on CLED plates varied from 1 cfu to 240 cfu. Five (5) bacterial isolates were identified from the urine samples and these isolates included; Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella sp. and Proteus sp. Aside from the S. aureus (U15), all the respective urine derived bacterial cultures exhibited susceptibility towards ciprofloxacin. All the bacterial strains were resistant to cloxacillin. S. aureus (US18) and Klebsiella sp. (U29) had maximal MAR value while S. aureus (U20) had the lowest MAR index value. The occurrence of female cases of asymptomatic bacteriuria within the relatively small population sub set should be reported to the relevant health care authorities within the institution for necessary action.

Item Type: Article
Subjects: R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Uchenna Eneogwe
Date Deposited: 27 May 2025 13:01
Last Modified: 27 May 2025 13:01
URI: http://eprints.gouni.edu.ng/id/eprint/4656

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