Assessment of the Pro12Ala Polymorphism in the PPAR-γ2 Gene among Type 2 Diabetes Patients in a Nigerian Population

Unachukwu, M. N and Engwa, G.A. and Nwalo, Friday Nweke and Chiezey, Venatus Osita (2018) Assessment of the Pro12Ala Polymorphism in the PPAR-γ2 Gene among Type 2 Diabetes Patients in a Nigerian Population. Journal of Clinical Medicine.

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Abstract

The association between the Pro12Ala polymorphism of the PPARγ2gene, type 2 diabetes (T2D),and obesity in certain ethnic populations has been reported. However, this relationship has not yet been described among diabetes patients in Nigeria. This study investigated the relationship between the Pro12Ala polymorphism in the PPARγ2 gene, obesity, and lipid abnormalities characterizing T2D among patients in Nigeria. This case-control study recruited 73T2D and 75non-diabetic(ND)patients. Demographic and clinical data were collected and blood glucose levels together with serum lipid profile for patients were measured. Pro12Ala polymorphism in the PPARγ2 gene was genotyped by restriction fragment length-Polymerase Chain Reaction(RFLP-PCR).The PPAR-γ2gene(amplicon size = 270base pair) was successfully amplified for all samples. Following restriction enzyme digestion and analysis by agarose gel electrophoresis, amplicons from samples showed a band of size 270 bp and were of the wild homozygous Pro/Progenotype. Ala12 variant was totally absent from the study population. Obesity, estimated using Body Mass Index (BMI) and waist circumference (WC), was significantly higher(p<0.05)in T2D patients compared to the non-diabetic patients. Moreso, the prevalence of lipid abnormalities;hypercholesterolaemia(TC>200mg/dL),hypertriglyceridaemia(TG>150mg/dL),high HDL(>100mg/dL),and lowHDL(<50mg/dL)was significantly greater(p<0.001)inT2Dpatients compared to non-diabetic patients. Results obtained further indicated lack of significant association betweenPPAR-γ2genepolymorphism,T2D,andobesity. However, obesity and dyslipidaemia were stronglyassociatedinT2Dpatients.

Item Type: Article
Subjects: L Education > L Education (General)
Q Science > Q Science (General)
Divisions: Faculty of Natural and Applied Sciences
Depositing User: GOUNI ICT
Date Deposited: 20 Jun 2018 18:19
Last Modified: 20 Jun 2018 18:19
URI: http://eprints.gouni.edu.ng/id/eprint/253

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