Ameliorative effect of imipenem on pulmonary damage caused by extended spectrum β-lactamase (ESBL) producing bacteria isolates from non- human source

Unachukwu, M. N and Afunwa, R. A and Iroha, R. I and Ugwu, M. (2018) Ameliorative effect of imipenem on pulmonary damage caused by extended spectrum β-lactamase (ESBL) producing bacteria isolates from non- human source. African Journal of Microbiology Research, 12 (15). pp. 354-361. ISSN 1996-0808

[img] Text
Ameliorative effect of imipenem on pulmonary damage caused by extended spectrum β-lactamase (ESBL) producing bacteria isolates from non- human source.pdf
Download (747kB)

Abstract

Acute lung infection induced by Extended Spectrum β-Lactamases (ESBL) producing isolates was determined by measuring inflammatory mediators; malondialdehyde (MDA), myeloperoxidase (MPO) and nitric oxide (NO). The mice were randomly divided into three groups of 20 animals each. All mice were given 104 c.f.u. ml-1 of the test organism intranasally in a volume of 50 µl while holding the mouse in an upright position without any anaesthesia. Group A received an intraperitoneal injection of an antibiotic, imipenem at a dose of 20 mg ml-1/ 25 g body weight which was administered 48 h post infection, Group B received only normal saline orally while group C, control-mice did not receive any treatment. The animals were sacrificed by cervical dislocation; lungs were removed aseptically and examined for various inflammatory mediators. The MDA, NO and MPO estimations in the lung homogenates in each group was measured and compared. Group treated with imipenem recorded lower absorbance values when compared with group treated with normal saline. The different parameters were statistically significant since the P-values were less than 0.05.

Item Type: Article
Subjects: L Education > L Education (General)
Q Science > Q Science (General)
Divisions: Faculty of Natural and Applied Sciences
Depositing User: GOUNI ICT
Date Deposited: 19 Jun 2018 12:29
Last Modified: 19 Jun 2018 12:29
URI: http://eprints.gouni.edu.ng/id/eprint/248

Actions (login required)

View Item View Item
GOUNI Repository is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.