In Vitro and In Vivo Antioxidant Activity of Aqueous and Ethanol Leaf Extracts of Murraya Koenigii

Engwa, G.A. and Unaegbu, M. and Francis, O.H and OBIUDU, I.K. and Agbafor, Kingsley N. and Ugwu, F.C (2016) In Vitro and In Vivo Antioxidant Activity of Aqueous and Ethanol Leaf Extracts of Murraya Koenigii. International Journal of Pharmacognosy and Phytochemical Research, 8 (4). pp. 551-557. ISSN 0975-4873

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Abstract

Reactive oxygen species generated from certain cellular processes cause tissue and cell damage and may alter the structure and functioning of proteins and nucleic acids. Endogenous antioxidant defence mechanisms involving certain enzymes as well as exogenous antioxidant from plants are capable of preventing such complications. This study investigated the in vitro and in vivo antioxidant activity of Murraya koenigii (curry leaf) extracts. Water and ethanol extracts of the leaf were obtained by soxhlet extraction. In vitro antioxidant activity was by the ferric reducing power and hydrogen peroxide scavenging assays and the total flavonoid and flavonol levels determined. The in vivo activity was assessed using albino wistar rats which were treated with 100mg/kg of aqueous and ethanol leaf extracts and ascorbic acid as positive control for six consecutive days after which a single dose (2.5mL/kg body) of CCl4 was administered except for the normal control group 24hrs later. The animals were sacrificed and the liver homogenate was used to assess the catalase activity and lipidic peroxidation. Aqueous and ethanol extracts showed ferric reducing activity and were concentration dependent. The hydrogen peroxide scavenging potential was very high at various concentrations for both extracts (between 81.2 to 99.9%) comparable to ascorbic acid. Molondialdehyde (MDA) level (lipidic peroxidation) was significantly lower (p0.05) in group 2 animals which received aqueous extract compared to group 1 (normal control) and group 4 (negative control). There was a significant difference (p0.05) in the catalase activity of group 2 animals which received aqueous extract compared to the normal control. Acute toxicity was observed in group 5 animals administered ethanol extract. Flavonoid and flavonol were greater in the ethanol extract (625.33 and 835.04 g/g of rutin) than in the aqueous extract (449.33 and 100.33 g/g of rutin) respectively. These results suggest that Murraya koenigii (curry leaf) aqueous and ethanol extracts possess antioxidant activity due to high amounts of flavonoid and flavonol. This property may partly be responsible for the medicinal application of the plant

Item Type: Article
Subjects: L Education > L Education (General)
Q Science > Q Science (General)
Divisions: Faculty of Natural and Applied Sciences
Depositing User: miss ifeoma agbo
Date Deposited: 18 Apr 2019 14:32
Last Modified: 18 Apr 2019 14:32
URI: http://eprints.gouni.edu.ng/id/eprint/1182

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