OXIDATIVE AND BIOCHEMICAL PARAMETER ASSESSMENT FOR ALLOXAN INDUCED DIABETIC RATS TREATED WITH METHANOL LEAF EXTRACTS OF OCIMUM GRATISSIMUM

OKECHUKWU, CHINWENDU OXIDATIVE AND BIOCHEMICAL PARAMETER ASSESSMENT FOR ALLOXAN INDUCED DIABETIC RATS TREATED WITH METHANOL LEAF EXTRACTS OF OCIMUM GRATISSIMUM. OXIDATIVE AND BIOCHEMICAL PARAMETER ASSESSMENT FOR ALLOXAN INDUCED DIABETIC RATS TREATED WITH METHANOL LEAF EXTRACTS OF OCIMUM GRATISSIMUM. (Unpublished)

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Abstract

Ocimum gratissimum is a vegetable plant of wide nutritional and medicinal application in Nigeria and some other parts of the world. The aim of this study is to know the effect and efficacy of oral administration of methanol extract of Ocimum gratissimum on the level of oxidative and biochemical parameters of alloxan-induced diabetic rats. Leaves of Ocimum gratissimum were obtained and dried under room temperature, after drying, it was grind into powder and 320.64g soaked in 50ml of methanol for 72hours. After which it was filtered with a Whatsman (no 1) filter paper. The filtrate was concentrated using rotary evaporator at 37oC. A total of 20 male Wistar albino rats were grouped into the normal group, diabetic not treated, diabetic treated with gilbenclamide and the group treated with the leaf extract, they were all fed orally with the extract for 14days. All the rats were sacrificed and their blood was collected for assaying of biochemical parameters. Result of the study showed a significant (P>0.05) increase in the serum SOD activity compared with the normal control.Vitamin E was observed showing a significant (P>0.05) increase in the group treated with the extracts compared with diabetic untreated. The result also showed a significant (P<0.05) decrease in MDA level of test group compared with the diabetic untreated and a non-significant (P>0.05) increase was seen in the HDL level of the test group and also a significant (P<0.05) decrease was observed in the LDL level of the group treated with leaf extract. Data of the study suggests that the oral administration of the aqueous methanol leaf extract of Ocimum gratissimum may impair naturally generated oxidant/toxicant activity and hereby enhance specific activities of antioxidants in rats.

Item Type:	Article
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Faculty of Natural and Applied Sciences
Depositing User:	Mrs Chinwendu Okechukwu Korie
Date Deposited:	31 Jul 2018 21:57
Last Modified:	31 Jul 2018 21:57
URI:	http://eprints.gouni.edu.ng/id/eprint/372

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