Isolation and identification of fungi from compost and garden soils

Mbah, Chinemerem (2018) Isolation and identification of fungi from compost and garden soils. Isolation and identification of fungi from compost and garden soils. (Unpublished)

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This project was designed for the isolation and identification of some fungi found in the soil. These microorganisms may be either pathogenic or non-pathogenic to the plants and humans. Two soil samples one from garden soil and the other one from compost soil were put into serial bags and taken to the laboratory for analysis. One gram of each sample was put into 9ml of serial water. Using pipette one in ten folds dilutions was done on each sample. Exactly 0.1 ml of each dilution was poured into Sabroaud dextrose agar plates and was spread using spreader on each Sabroaud dextrose agar plates and left on the table for 24 to 48hrs. The representative growth on each plate was sub-cultured on Sabroaud dextrose agar slant to obtain pure culture for identification and left for at least 72hrs for spores to be formed. Slide culture technique was used for proper identification of the filamentous fungi. Lacto phenol blue was used to stain the growth on the slide and cover slip and viewed under the microscopy. A total of three fungal isolates were obtained from the soil samples on the Sabroaud dextrose agar plates. Three fungi species, were identified and from the fungal isolates species belonging to the genera Aspergillus spp and Mucor sp. The identified soil fungi from the two soil sample were Aspergillus niger, Aspergillus fumigatus and Mucor sp. and the three were isolated from two soil samples. In this result fungi growth occur more in compost soil than in garden soil and the most common organism was Aspergillus spp. The two soil fungi obtained in this work were known to be pathogenic to human beings. Some Aspergillus spp are known to produce mycotoxic which can cause disease in man e.g. Aflatoxins. It is a cumulative toxins. Also Mucor sp are known to cause disease in man through inhalation of the spores in the air e.g. systematic mucormycosis in diabetic patients.

Item Type: Article
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Natural and Applied Sciences
Depositing User: Article Jennifer Mbah
Date Deposited: 31 Jul 2018 21:58
Last Modified: 31 Jul 2018 21:58

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