DNA barcoding for identifcation of fish species from freshwater in Enugu and Anambra States of Nigeria

Ude, George N. and Igwe, David O. and Chrysten, Brown and Myron, Jackson and Alusine, Bangura and Ozokonkwo‑Alor, Onyinye and Ihearahu, Onyinye C. and Obih, Chosen and Okoro, Michael and Ene, Christabel and Chieze, Venatus and Unachukwu, M. N and Onyia, Christie and Acquaah, George and Ogbonna, James and Das, Aditi (2020) DNA barcoding for identifcation of fish species from freshwater in Enugu and Anambra States of Nigeria. METHODS AND RESOURCES ARTICLE, 12. pp. 643-658.

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Within Enugu and Anambra States, Nigeria, identifcation of fshes has been based on morphological traits and do not account for existing biodiversity. For DNA barcoding, assessment of biodiversity, conservation and fshery management, 44 fsh sampled from Enugu and Anambra States were isolated, amplifed and sequenced with mitochondrial cytochrome oxidase subunit I (COI). Twenty groups clustering at 100% bootstrap value including monophyletic ones were identifed. The phylogenetic diversity (PD) ranged from 0.0397 (Synodontis obesus) to 0.2147 (Parachanna obscura). The highest percentage of genetic distance based on Kimura 2-parameter was 37.00±0.0400. Intergeneric distances ranged from 15.8000 to 37.0000%. Congeneric distances were 6.9000±0.0140–28.1000±0.0380, with Synodontis as the existing synonymous genus. Confamilial distances in percentage were 16.0000±0.0140 and 25.7000±0.0300. Forty-two haplotypes and haplotype diversity of 0.9990±0.0003 were detected. Nucleotide diversity was 0.7372, while Fu and Li’s D* test statistic was 2.1743 (P<0.02). Tajima’s D was 0.2424 (P>0.10) and nucleotide frequencies were C (17.70%), T (29.40%), A (24.82%), G (18.04%) and A +T (54.22%). Transitional mutations were more than transversions. Twenty species (99–100%) were identifed with the e-value, maximum coverage and bit-score of 1e−43, 99–100 and 185–1194, respectively. Seventeen genera and 12 families were found and Clariidae (n=14) was the most dominant among other families. The fsh species resolution, diversity assessment and phylogenetic relationships were successfully obtained with the COI marker. Clariidae had the highest number of genera and families. Phylogenetic diversity analysis identifed Parachanna obscura as the most evolutionarily divergent one. This study will contribute to fshery management, and conservation of freshwater fshes in Enugu and Anambra States, Nigeria.

Item Type: Article
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculty of Natural and Applied Sciences
Depositing User: mrs chioma hannah
Date Deposited: 23 Nov 2020 08:17
Last Modified: 23 Nov 2020 08:17
URI: http://eprints.gouni.edu.ng/id/eprint/2867

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